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Journal 'Cytokines & inflammation', 2004, No. 4

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Original Articles

Number 4'2004

A MODEL OF DELAYED WOUND HEALING IN GLUCOCORTICOID-TREATED IMMUNOCOMPROMISED MICE

E.A. Variouchina, M.A. Antsiferova, G.V. Aleksandrov, E.N. Minaeva, N.V. Pigareva, A.V. Petrov, V.G. Konusova, E.N. Isaeva, V.E. Bokovanov, A.Yu. Kotov, A.A. Kazakov, A.V. Demjanov, A.S. Simbirtsev

Systemic application of glucocorticoids can cause severe defects in wound healing due to their anti-inflammatory and anti-proliferative activity. With the purpose of developing a model of delayed wound healing, hydrocortisone (25 mg/kg) was injected i.m. daily during 14 days; two full-thickness wounds were created in each mouse a day after the first injection of hydrocortisone. The wound surface areas were measured at several time points and wounded sites were excised for histological evaluation. Immune parameters, such as spleen weight, number of spleen lymphocytes, and number of leukocytes, lymphocytes and neutrophils and peripheral blood neutrophils chemiluminescence were assessed. The results obtained showed a statistically significant delay of wound healing in hydrocortisone-treated mice. Histological examination indicated decrease in re-epithelization of wound surfaces and granulation tissue formation. The analysis of immune parameters revealed significant decrease of body and spleen weights and number of lymphocytes in peripheral blood and spleen. The model can be used to study the therapeutic potency of pharmacological preparations such as recombinant cytokines for delayed wound healing treatment. (Cytokines and Inflammation. 2004. Vol. 3, N 4. P. 14-20.)

Keywords: delayed wound healing, glucocorticoids, immunosuppression.

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