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Journal 'Cytokines & inflammation', 2012, No. 2

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Original Articles

Number 2'2012

Cytokine-dependent mechanisms of Staphylococcus aureus Cowan I phagocytosis

S.V. Slobodchikova, K.V. Shmagel

Phagocytosis is the main host defense mechanism against Staphylococcus aureus Cowan I. This process is regulated by cytokines, opsonins, and antimicrobial molecules. We investigated the ability of inactivated S. aureus Cowan I to induce the synthesis of TNFα, IL-1β and IL-1Ra in vitro depending on the bacteria concentration. 24-hour whole blood cultures were used. In addition, the supernatants of cultures activated by staphylococci (SAC) were tested in the phagocytic reactions with S. aureus Cowan I. It was shown that at low concentrations of the microorganisms IL-1Ra production exceeded the production of IL-1β. The increasing of the bacterial concentration in the cultures led to the situation when the synthesis of IL-1β exceeded the level of IL-1Ra production. The induction of TNFα formation required fewer S. aureus Cowan I than was necessary for the production of IL-1β. SAC significantly increased the engulfment of S. aureus Cowan I by neutrophils. An enhancement of phagocyte bactericidal activity was less pronounced and was associated with cytokine priming. The last was confirmed by the experiments with pentoxifylline: its presence in the cultures abolished the amplifying effect of SAC on luminol-dependent chemiluminescence. However, despite the positive influence of SAC, the bactericidal response of phagocytes against S. aureus Cowan I remained relatively low: the uptake of zymosan by leukocytes accompanied by more intense (8–10 times) generation of active radicals by phagocytes. (Cytokines and Inflammation. 2012. Vol. 11. № 2. P. 107–112.)

Keywords: Staphylococcus aureus.

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