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Journal 'Cytokines & inflammation', 2012, No. 2

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Original Articles

Number 2'2012

SOCS1 expression in peripheral blood mononuclears of patients with bronchial asthma

L.N. Sorokina, V.N. Mineev, V.V. Lim, M.А. Nyoma, V.I. Trofimov

14 healthy controls, 40 patients with allergic bronchial asthma (ABA) and 33 with non-allergic bronchial asthma (NABA) were examined. The expression of SOCS1 mRNA was analyzed by RT-PCR. Primers of SOCS1 have been developed on the basis of known sequences (GenBank). Expression level of SOCS1 mRNA was estimated relatively to the level of β-actin mRNA. Preparation of cell lysates, and the Western blotting were performed through the standard procedure. Anti-SOCS1 antibodies were used. The level of protein was analyzed relatively to β-actin using anti-actin antibodies. We determined the decrease (5,4-fold) of SOCS1 expression in peripheral blood mononuclears in patients with ABA in comparison with healthy persons (p=0,036) and NABA patients (5,3-fold) (p=0,024) (U-criterion). NABA patients had no significant difference in comparison with healthy persons. SOCS1 mRNA expression level in peripheral blood mononuclears in ABA patients was significantly lower than in the healthy persons (1,4 times) (p=0,048; Tukey criterion) and NABA patients (1,3 times) (p=0,041; Tukey crit.). SOCS1 may play an important role in pathogenesis of bronchial asthma. Decreased expression of SOCS1 protein, and the level of SOCS1 mRNA expression in patients with ABA may indicate a defect in the system of negative regulation in atopic asthma. (Cytokines and Inflammation. 2012. Vol. 11. № 2. P. 23–27.)

Keywords: bronchial asthma, SOCS- proteins system, SOCS1 protein, mononuclear cells.

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