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Journal 'Cytokines & inflammation', 2004, No. 1
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Original Articles
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Number 1'2004
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THE ASSESSMENT OF CYTOKINE PATTERN IN PATIENTS WITH SEVERE SEPSIS USING THE BIO-PLEX-PROTEIN ASSAY SYSTEM
A.A. Ostanin, O.Yu. Leplina, E.Ya. Shevela, E.R. Chernykh, V.I. Konenkov
The goal of current study was the multiplex quantitative measurement of 18 cytokines in sera of patients with severe sepsis, including septic shock. The development of combined T-cell/monocyte immune disorders due to the cytokine balance shift following the increase of immunosupressive and anti-inflammatory mediators was revealed in patients (n = 14) with severe sepsis. The measurement of cytokines using ELISA and Bio-Plex assay showed that in contrast to healthy donors the septic patients were characterized by increased serum level of IL-1Ra, total IL-10, IL-6 and MIP-1b, whereas the concentrations of pro-inflammatory cytokines (IL-1b, TNFb, IL-12, IL-17) were near-normal. The elevated levels of IL-8 (50 % of cases), MCP-1 (28,6 %), GM-CSF (35,7 %), and G-CSF (21,4 %) were detected in some patients. The high concentrations of IL-1Ra, total IL-10 and MIP-1b were associated with severity indexes including Acute Physiology And Chronic Health Evaluation (APACHE-II) and Sepsis-related Organ Failure Assessment (SOFA) scores. It was also shown that the septic shock patients were characterized by 1) more prominent depression of T-cell proliferative response in vitro, 2) marked decrease of HLA-DR expression in monocytes, 3) enhanced supressive and anti-inflammatory activities of serum in a bioassay, and 4) higher level of IL-1Ra, total IL-10, IL-6, IL-8, G-CSF, GM-CSF, MCP-1 and MIP-1b. Our data indicate the predominance of anti-inflammatory/immunosupressive reactions in patients with severe sepsis that are "masked" by the clinical signs of systemic inflammatory response syndrome (SIRS). (Cytokines and Inflammation. 2004. Vol. 3, N1. P. 20-27.)
Keywords: cytokines, Bio-Plex, sepsis, septic shock, multiorgan deficiency syndrome.
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