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Journal 'Cytokines & inflammation', 2015, No. 1

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Original Articles

Number 1'2015

The expression of membrane-bound TNFα receptors on monocytes in atopic dermatitis and rheumatoid arthritis

A.A. Alshevskaya, Yu.A. Lopatnikova, N.S. Shkaruba, O.A. Chumasova, A.E. Sizikov, O.L. Krugleeva V.M. Nepomnyashchikh, F.F. Vasilyev, S.V. Sennikov

Tumor necrosis factor alpha (TNFα) plays a critical role in rheumatoid arthritis and atopic dermatitis. Monocytes are actively involved in the inflammation in both pathologies, and the effects of mediator largely depend on the absolute number of membrane-bound receptors. Objective: to evaluate expression of TNFα membrane-bound receptors both by the percentage of monocytes expressing these receptors and the number of receptor molecules expressed on monocytes with and without LPS stimulation in patients with different immunopathology. Methods and materials. The objects of the study were PBMCs cultured overnight with or without LPS. The phenotypic characterization of monocytes was performed by flow cytometry. To determine number of receptors per cell Quantibrite PE Beads were used. Results: density of TNFα receptor expression on the monocyte surface is higher in patients with atopic dermatitis as well as in patients with rheumatoid arthritis compared to healthy donors and reflects cell response to the activation depending upon the health condition and disease stage. Conclusion: during inflammatory diseases with various etiologies changes are formed in TNFα membrane-bound receptors on the cell surface which are implemented changes not only through changes in percent positive cells, but also the density of receptor expression, reflecting the different embodiments of the regulation of cytokine biological properties by targeted cells. (Cytokines and Inflammation. 2015. Vol. 14. № 1. P. 18–23.)

Keywords: tumor necrosis factor-alpha, membrane-bound receptors, density of receptor expression, rheumatoid arthritis, atopic dermatitis.

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