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Journal 'Cytokines & inflammation', 2015, No. 3

Orexin A- and orexin B-containing neurons in the rat hypothalamus after lipopolysaccharide injection

V.A. Pugach, S.V. Perekrest, N.S. Novikova, E.A. Korneva

Aim: to study participation of hypothalamic orexin A- and orexin B-containing neurons in brain responses to lipopolysaccharide (LPS) administration. 1 hr after LPS or saline (control) injection animals were perfused intracardially for further immunohistochemical detection of orexin-positive neurons in hypothalamic structures (dorsomedial nucleus (DMH), regions of lateral hypothalamic area: juxtadorsomedial (LHAjd), suprafornical (LHAs) and dorsal (LHAd)). Our data suggest that orexin A- and orexin B-containing neurons respond to antigen administration by changes of their quantity and relative optical density (ROD). 1 hr after LPS injection orexin A-containing neurons quantity was increased only in LHAs, and orexin-B-containing neurons quantity — in DMH, LHAjd, LHAs. ROD of orexin-A-positive neurons was decreased in all investigated structures. Orexin B-containing neurons showed different reaction in response to antigen injection: their ROD was decreased in DMH, but increased in LHAjd and LHAs. Thus, we can conclude that the population of orexin-containing neurons is functionally heterogeneous because neurons localized in various hypothalamic structures respond differently to LPS administration. (Cytokines and Inflammation. 2015. Vol. 14. № 3. P. .)

Keywords: neuroimmune interaction, hypothalamus, orexin A and B, lipopolysaccharide.

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